Chinese Journal of Tissue Engineering Research ›› 2013, Vol. 17 ›› Issue (20): 3730-3737.doi: 10.3969/j.issn.2095-4344.2013.20.017
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Situ Yong-li, Xie Ai-mei, Zheng Zhi-ming, Lu Han-yun, Wu Tie
Received:
2012-10-18
Revised:
2012-12-06
Online:
2013-05-14
Published:
2013-05-14
Contact:
Wu Tie, Master, Professor, Master’s supervisor, Department of Pharmacology, Pharmacy College, Guangdong Medical College, Zhanjiang 524023, Guangdong Province, China
wutie2@163.com
About author:
Situ Yong-li★, Studying for master’s degree, Department of Pharmacology, Pharmacy College, Guangdong Medical College, Zhanjiang 524023, Guangdong Province, China
562004233@qq.com
Supported by:
Science and Technology Plan Projects of Guangdong Province, No. 2010B06050
CLC Number:
Situ Yong-li, Xie Ai-mei, Zheng Zhi-ming, Lu Han-yun, Wu Tie. Ruanmai preparation prevents vascular calcification in mice [J]. Chinese Journal of Tissue Engineering Research, 2013, 17(20): 3730-3737.
2.1 实验动物数量分析 纳入动物数量40只,分4组进行实验,其中普伐他汀钠组有1只、维生素D3+高脂乳剂模型组有1只小鼠因维生素D3急性中毒而死,其余小鼠存活至实验结束,进入结果分析38只。 2.2 胸主动脉大体观察与组织学观察 胸主动脉的大体观察:正常对照组胸主动脉血管外壁光滑,呈淡粉色半透明状,柔软而富有弹性,触之光滑无沙粒感,管壁厚度均匀。模型组胸主动脉血管外壁凹凸不平,团块状灰白色突起弥漫性分布,管壁僵硬易断,触之有沙粒感,管壁厚薄不均。普伐他汀钠组胸主动脉血管外壁凹凸不平,团块状灰白色突起弥漫性分布,有沙粒感,管壁厚薄不均。“软脉1号”制剂组比模型组有所改善,胸主动脉血管外壁凹凸不平减轻,团块状灰白色突起弥漫性分布减少,有沙粒感,管壁厚薄比较均匀。 胸主动脉的镜下观察:正常对照组观察可见胸主动脉血管壁结构清晰,血管内中外膜分界清楚,内皮细胞完整、突向管腔排布,内弹性膜与中层弹性纤维均呈粉染、清晰,波浪状走向,弹性纤维间平滑肌细胞呈梭形,排列整齐而均匀,管壁无黑色钙质沉积,内外弹性膜结构完整,见图1。 模型组观察可见胸主动脉血管内皮细胞消失,有大量黑色钙质沉积于中层弹性纤维中,管壁弹性纤维间距扩大,弹性纤维结构模糊、分辨不清,走向变直,部分断裂,平滑肌细胞坏死消失,内外弹性膜结构不完整,见图2。"
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